• If the blood sample is collected to measure alcohol content, clean the site of injection with saline swab and not a spirit swab.
• EDTA should be used as anticoagulant. Tubes with liquid EDTA /fluoride reduce the risk of hemolysis which may alter results.
• Draw about 3–4 ml of blood sample into chemically clean evacuated tubes of 5ml. Use labels with identification codes to mark the tubes. EDTA should be used as an anticoagulant. Tubes with liquid EDTA /fluoride reduce the risk of hemolysis that may change results.
• Add 2ml of 5% aqueous solution of sodium citrate containing 0.2% w/v of formaldehyde or 0.5% w/v of formalin solution to prevent decomposition, which may alter results.
• Plastic vacuum tubes/ Plastic vacuum gel tubes are preferred to glass tubes. If vacuum tubes are not available or tubes are opened for freely flowing samples, stoppers which do not react with blood constituents should be available.
• Special boxes for tube transfer and storage, earmarked refrigerator/freezer must be available in hospital dealing with medicolegal cases.
• Two, approximately 1cm x 1cm size, blood stains are formed on clean cotton cloth/gauze pieces and, after they are dry, they are transferred to a sterile 10ml injection vial. It should be properly dried, before packing to avoid decomposition and then sealed and labeled.
• Bloodstains located on the body of an uninjured person are taken by rubbing with moistened clean cotton cloth pieces, it should be properly dried, before packing to avoid decomposition and then sealed and labeled.
(Contributed by Dr Sudhir Gupta)
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